Research Assistant - Protein Expression & Process Development

About KisoJi Biotech

KisoJi Biotech is advancing next-generation antibody therapeutics through the integration of proprietary transgenic heavy chain-only antibody (HCAb) platforms, AI-guided discovery, and high-throughput protein production systems.

Position Overview

Position: Research Assistant

Site: Montréal, Quebec, Canada

Department: Process Development

We are currently expanding our protein sciences capabilities and are seeking a Research Assistant to support our recombinant protein expression and purification workflows across multiple expression systems including mammalian (HEK293, CHO/ExpiCHO), insect (Sf9/Hi5), and bacterial (E. coli) hosts.

This is a hands-on laboratory role focused on supporting the development and optimization of protein expression and downstream purification processes to advance antibody discovery, functional screening, and structural biology programs. The position will involve working closely with a multidisciplinary team spanning molecular biology, antibody engineering, AI-guided discovery, and analytical characterization.

The successful candidate will demonstrate strong laboratory discipline, a willingness to learn, and a genuine enthusiasm for contributing to the development of next-generation antibody therapeutics.

Qualifications & ResponsibilitiesEducation & Experience

· B.Sc. in Molecular Biology, Biochemistry, or a related field; M.Sc. considered an asset

· Prior laboratory experience through academic coursework, internships, or co-op placements is preferred

· Familiarity with standard molecular biology and protein biochemistry techniques is an asset

Core Technical Responsibilities

· Assist in recombinant protein expression experiments across mammalian (HEK293, CHO/ExpiCHO), insect (Sf9/Hi5), and bacterial (E. coli) expression systems

· Support cell culture maintenance and cell line development workflows for antibody production

· Perform protein purification using FPLC-based chromatography systems (affinity, ion exchange, size exclusion)

· Assist with basic biophysical characterization of purified proteins to assess quality and homogeneity

· Maintain detailed and accurate laboratory notebooks, records, and standard operating procedures (SOPs)

· Assist with general laboratory operations including reagent preparation, equipment maintenance, and inventory management

Collaboration & Teamwork

· Work under the direction of senior scientists and research associates to support ongoing protein production programs

· Participate in team meetings, contribute observations, and communicate experimental progress clearly

· Foster a collaborative and respectful lab environment consistent with KisoJi's scientific values

Professional Competencies

· Strong attention to detail and commitment to accuracy and reproducibility in the laboratory

· Eagerness to develop technical skills and grow within a fast-paced, science-driven organization

· Good written and oral communication skills in English; French language skills are an asset in the Montréal environment

· Ability to follow protocols precisely, manage assigned tasks, and meet deadlines

· Works well both independently and as part of a team

What We OfferBenefits

· Comprehensive health and dental insurance plans

· Paid vacation and statutory holidays

Growth & Development

· Structured on-the-job training and professional development programs

· Annual performance reviews with merit-based bonuses

· Opportunity to work with cutting-edge, proprietary antibody discovery technologies

· Direct exposure to advanced AI-guided therapeutic discovery platforms

How to Apply

Interested candidates are invited to submit a cover letter outlining their fit for this position, along with a current CV, to: ***email_hidden***

Please include the position title, department, and site location in the subject line of your email. Applications submitted through any other channel will not be considered. Shortlisted candidates will be asked to provide professional references and a detailed skills inventory.

We thank all applicants for their interest; only those selected for an interview will be contacted.